Facilitation by acetylcholine of tetrodotoxin-resistant spikes in rat hippocampal pyramidal cells
Identifieur interne : 001800 ( Main/Exploration ); précédent : 001799; suivant : 001801Facilitation by acetylcholine of tetrodotoxin-resistant spikes in rat hippocampal pyramidal cells
Auteurs : B. H. G Hwiler [Suisse]Source :
- Neuroscience [ 0306-4522 ] ; 1984.
English descriptors
Abstract
Abstract: The electrical activity of hippocampal pyramidal cells was studied in slice cultures during blockade of the regenerative Na currents. In the presence of tetrodotoxin, these neurones had a mean resting potential of −68 mV, a membrane input resistance of 87 MΩ and displayed marked non-linearities in their current voltage relationship. In response to depolarizing stimuli, pyramidal cells generated action potentials of small amplitude, slow rise and long duration. These tetrodotoxin-resistant spikes were abolished by calcium conductance blockers such as cobalt and cadmium ions.Acetylcholine applied to the bath or by iontophoresis depolarized pyramidal cells, elicited spontaneous tetrodotoxin-resistant spikes and facilitated spiking evoked by depolarizing rectangular current pulses or a current ramp. The effects of acetylcholine were not only slow in onset, but also prolonged; they were completely reversible and sensitive to atropine and calcium-antagonists such as cadmium and cobalt ions which, respectively, reduced and abolished these effects. Afterhyperpolarizations following injection of depolarizing current pulses were suppressed by acetylcholine and often transformed into depolarizing afterpotentials.Acetylcholine had no effect on voltage-independent conductances as determined by application of hyperpolarizing current pulses. These results could be explained by inhibition of the voltage-dependent K+-current, i.e. the M current (blockade of the calcium current could remove any depolarizing influence resulting from M current inhibition) or by a direct activation of a voltage-dependent calcium current by muscarinic agonists.
Url:
DOI: 10.1016/0306-4522(84)90030-7
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000783
- to stream Istex, to step Curation: 000450
- to stream Istex, to step Checkpoint: 000F61
- to stream Main, to step Merge: 001860
- to stream Main, to step Curation: 001800
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Facilitation by acetylcholine of tetrodotoxin-resistant spikes in rat hippocampal pyramidal cells</title><author><name sortKey="G Hwiler, B H" sort="G Hwiler, B H" uniqKey="G Hwiler B" first="B. H." last="G Hwiler">B. H. G Hwiler</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D81D96DE7409AE4A5493071138D4D8914542AFF1</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1016/0306-4522(84)90030-7</idno><idno type="url">https://api.istex.fr/document/D81D96DE7409AE4A5493071138D4D8914542AFF1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000783</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000783</idno><idno type="wicri:Area/Istex/Curation">000450</idno><idno type="wicri:Area/Istex/Checkpoint">000F61</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F61</idno><idno type="wicri:doubleKey">0306-4522:1984:G Hwiler B:facilitation:by:acetylcholine</idno><idno type="wicri:Area/Main/Merge">001860</idno><idno type="wicri:Area/Main/Curation">001800</idno><idno type="wicri:Area/Main/Exploration">001800</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Facilitation by acetylcholine of tetrodotoxin-resistant spikes in rat hippocampal pyramidal cells</title><author><name sortKey="G Hwiler, B H" sort="G Hwiler, B H" uniqKey="G Hwiler B" first="B. H." last="G Hwiler">B. H. G Hwiler</name><affiliation wicri:level="1"><country xml:lang="fr">Suisse</country><wicri:regionArea>Preclinical Research, Sandoz Ltd., CH 4002 Basel</wicri:regionArea><wicri:noRegion>CH 4002 Basel</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Neuroscience</title><title level="j" type="abbrev">NSC</title><idno type="ISSN">0306-4522</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1984">1984</date><biblScope unit="volume">11</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="381">381</biblScope><biblScope unit="page" to="388">388</biblScope></imprint><idno type="ISSN">0306-4522</idno></series><idno type="istex">D81D96DE7409AE4A5493071138D4D8914542AFF1</idno><idno type="DOI">10.1016/0306-4522(84)90030-7</idno><idno type="PII">0306-4522(84)90030-7</idno><idno type="ArticleID">84900307</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0306-4522</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ACh</term><term>TTX</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The electrical activity of hippocampal pyramidal cells was studied in slice cultures during blockade of the regenerative Na currents. In the presence of tetrodotoxin, these neurones had a mean resting potential of −68 mV, a membrane input resistance of 87 MΩ and displayed marked non-linearities in their current voltage relationship. In response to depolarizing stimuli, pyramidal cells generated action potentials of small amplitude, slow rise and long duration. These tetrodotoxin-resistant spikes were abolished by calcium conductance blockers such as cobalt and cadmium ions.Acetylcholine applied to the bath or by iontophoresis depolarized pyramidal cells, elicited spontaneous tetrodotoxin-resistant spikes and facilitated spiking evoked by depolarizing rectangular current pulses or a current ramp. The effects of acetylcholine were not only slow in onset, but also prolonged; they were completely reversible and sensitive to atropine and calcium-antagonists such as cadmium and cobalt ions which, respectively, reduced and abolished these effects. Afterhyperpolarizations following injection of depolarizing current pulses were suppressed by acetylcholine and often transformed into depolarizing afterpotentials.Acetylcholine had no effect on voltage-independent conductances as determined by application of hyperpolarizing current pulses. These results could be explained by inhibition of the voltage-dependent K+-current, i.e. the M current (blockade of the calcium current could remove any depolarizing influence resulting from M current inhibition) or by a direct activation of a voltage-dependent calcium current by muscarinic agonists.</div></front></TEI><affiliations><list><country><li>Suisse</li></country></list><tree><country name="Suisse"><noRegion><name sortKey="G Hwiler, B H" sort="G Hwiler, B H" uniqKey="G Hwiler B" first="B. H." last="G Hwiler">B. H. G Hwiler</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CobaltMaghrebV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001800 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001800 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= CobaltMaghrebV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:D81D96DE7409AE4A5493071138D4D8914542AFF1
|texte= Facilitation by acetylcholine of tetrodotoxin-resistant spikes in rat hippocampal pyramidal cells
}}
| This area was generated with Dilib version V0.6.32. |  |